hga030hga050怎么注册_HGA无形体IgG抗体检测试剂盒（IFA荧光玻片法）

皇冠信用网开户(www.mos011.vip）-会员开户_登3代理）皇冠信用网/—开会员账号—皇冠信用网招登1登2登3皇冠信用网足球平台出租）【产品规格】120T/盒

【保存温度】

12口井的10张幻灯片hga030hga050怎么注册。每个孔都含有感染嗜吞噬细胞的灭活细胞。密封的载玻片在载玻片上注明的日期之前是稳定的在2至8°C的温度下储存时，包装标签。为了避免冷凝，在打开密封的包装之前，让载玻片加热到室温。

【预期用途】

Focus Diagnostics Anaplasma吞噬细胞间接免疫荧光抗体（IFA）IgG检测试剂盒检测半定量人血清中针对吞噬细胞无浆细胞的IgG类抗体，作为人类粒细胞无浆体病（HGA）诊断的检测方法hga030hga050怎么注册。

【检测原理】

间接免疫荧光抗体（IFA）检测是一个两阶段的“三明治”程序hga030hga050怎么注册。在第一阶段，患者血清在PBS中稀释将血清放置在与底物接触的载玻片上并孵育。孵育后，将载玻片在PBS中洗涤，以去除未结合的血清抗体。在第二阶段，每个抗原孔都覆盖有荧光素标记的人IgG抗体。将载玻片孵育，使抗原抗体复合物与荧光素标记的抗人IgG反应。在清洗、干燥和安装载玻片后，使用荧光显微镜进行检查。阳性反应表现为胚的苹果绿色荧光。半定量终点滴度是通过检测阳性的连续稀释液获得的标本。

无形体IFA抗体检测试剂盒

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TEST PRINCIPLE

The indirect immunofluorescent antibody (IFA) assay is a two stage “sandwich” procedure. In the first stage, the patient serum is diluted in PBS. The diluted

serum is placed on the slide in contact with the substrate, and incubated. Following incubation, the slide is washed in PBS, which removes unbound serum

antibodies. In the second stage, each antigen well is overlaid with fluorescein-labeled antibody to human IgG. The slide is incubated allowing antigen antibody

complexes to react with the fluorescein-labeled anti-human IgG. After the slide is washed, dried, and mounted, it is examined using fluorescence microscopy.

Positive reactions appear as apple-green fluorescence of the morulae. Semi-quantitative endpoint titers are obtained by testing serial dilutions of positive

specimens.